1. Currently, the method of choice for endotoxin detection is the Limulus Amebocyte Lysate ( LAL ) test. 2. His " Limulus " specimens can still be seen in the Grant Museum of Zoology at UCL today. 3. Very low levels of LPS can cause coagulation of the limulus lysate due to a powerful amplification through an enzymatic cascade. 4. He was the first to show the relationship of the horseshoe crab or " Limulus " to the Arachnida. 5. For example, several studies have looked into the effect of a circa tidal rhythm on the locomotion of " Limulus polyphemus" 6. The standard assay for detecting presence of endotoxin is the Limulus Amebocyte Lysate ( LAL ) assay, utilizing blood from the Horseshoe crab. 7. The study found that light and dark cycles influence locomotion in " Limulus polyphemus, " but not as much as tidal activity. 8. From this basic research, a reagent, Limulus amoebocyte lysate ( LAL ), was developed that can detect minute amounts of bacterial toxins. 9. Sublensar sensory structures have been found in the eyes of some apposition eyes, especially " Limulus ", a genus of horseshoe crabs. 10. The protein hemocyanin is the oxygen carrier in most mollusks and some arthropods such as the horseshoe crab ( " Limulus polyphemus " ). 
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