1. Common test organisms include, but are not limited to, daphnia, fathead minnow, rainbow trout, and mussels. 2. Many vitamins and minerals are vital for the correct function of cells in the test organism . 3. To perform the OF-glucose test, two tubes of OF-glucose medium are inoculated with the test organism . 4. The test organisms are exposed for 48 hours under static conditions with five concentrations of the effluent. 5. Many of these methods use the same test organism or are designed for the same exposure time. 6. Often pilot studies are utilized to assist in the selection of the appropriate test organism and end points. 7. The rapid growth of duckweeds finds application in bioremediation of polluted waters and as test organisms for environmental studies. 8. The researchers, headed by Dr . E . Peter Greenberg of Iowa, used the Pseudomas microbe as their test organism . 9. To conduct such a screen, isolates of many different microorganisms are diffusible products that inhibit the growth of test organisms . 10. On the other hand, they may require considerable time and labour, and the number of test organisms is often relatively limited. 
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