31. Sequencing by ligation relies upon the sensitivity of DNA ligase for base-pairing mismatches. 32. This leaves behind a nicked DNA strand that is sensed and ligated by DNA Ligase . 33. The molecular weight of o-succinylbenzoate CoA ligase is 185000 Da or 185 kDa. 34. It is formed by glutamate cysteine ligase and used by glutathione synthetase to form glutathione. 35. Parkin is a cytosolic E3 ubiquitin ligase . 36. Ligase III is upregulated in chronic myeloid leukemia, multiple myeloma, and breast cancer.37. These ends are called cohesive since they are easily joined back together by a ligase . 38. DNA ligase from eukaryotes and some microbes uses adenosine triphosphate ( ATP ) rather than NAD. 39. Most experiments use T4 DNA Ligase ( isolated from overhang, the lower the T m. 40. This experiment further supported the Okazakis'hypothesis of discontinuous replication and linkage by polynucleotide ligase . 